The Hepworth Lab

GUS staining protocol for whole mount and sectioning

Phloroglucinol staining for lignin analysis

Floral dip protocol for plant transformation

Making electrocompetent Agro and transformation protocol

Taq polymerase purification

Tang's magic PCR buffer for Taq polymerase

Genomic DNA isolation for PCR genotyping plants

Selecting for transformed seeds on kanamycin plates

Hygromycin selection protocol for plants

Surface sterilization of seeds

72-cell plant tray scoring grid

In situ hybridization

• Fixation and pouring wax molds

• Fixing in situ slides

• In situ carbonate hydrolysis

• Making the in situ probe

• In situ hybridization protocol